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HOHSAKA, Takahiro Professor, Director of Bioscience, Biotechnology, and Biomedical Engineering Research Area
Materials Science, Bioscience, Biotechnology, and Biomedical Engineering
◆Degrees
M.E.(1994) and Ph.D.(2002) from Tokyo Institute of Technology 東京工業大学
◆Professional Experience
: Research Associate, Okayama University (1994-2003), PRESTO Researcher, Japan Science and Technology Agency(2002-2006)
◆Specialties
Molecular biochemistry
◆Research Keywords
Nonnatural amino acid, Cell-free translation, Artificial protein, Four-base codon, Fluorescence, Biological Organic Chemistry
◆Research Interests
Incorporation of Nonnatural Amino Acids into Proteins through Extension of the Genetic Code
We have developed a novel system that allows us to introduce "nonnatural amino acids" into specific positions of proteins by using "four-base codons". Using this system, the following studies are in progress.- Structural and functional analysis of proteins by introducing fluorescent amino acids- Improvement of technology for nonnatural amino acid incorporation- Incorporation of modified amino acids into proteins- Development of novel site-directed mutagenesis technology

■Publications

◆Published Papers
Antigen-responsive fluorescent antibody probes generated by selective N-terminal modification of IgGs
Fukunaga Keisuke, Watanabe Takayoshi, Novitasari Dian, Ohashi Hiroyuki, Abe Ryoji, Hohsaka Takahiro
CHEMICAL COMMUNICATIONS, 54, 90, 12734-12737, 2018
Antigen-dependent fluorescence response of anti-c-Myc Quenchbody studied by molecular dynamics simulations
Yoshiharu Mori, Hisashi Okumura, Takayoshi Watanabe, Takahiro Hohsaka
Chemical Physics Letters, 698, 223-226, 2018
Statistical description of the denatured structure of a single protein, staphylococcal nuclease, by FRET analysis
Mariko Yamaguchi, Emi Ohta, Takuya Muto, Takayoshi Watanabe, Takahiro Hohsaka, Yoichi Yamazaki, Hironari Kamikubo, Mikio Kataoka
Biophysical Reviews, 10, 2, 145-152, 2018
Freezing-Assisted Gene Delivery Combined with Polyampholyte Nanocarriers
Sana Ahmed, Tadashi Nakaji-Hirabayashi, Takayoshi Watanabe, Takahiro Hohsaka, Kazuaki Matsumura
ACS BIOMATERIALS SCIENCE & ENGINEERING, 3, 8, 1677-1689, 2017
Novel biosensor system model based on fluorescence quenching by a fluorescent streptavidin and carbazole-labeled biotin
Xianwei Zhu, Hiroaki Shinohara, Ryuta Miyatake, Takahiro Hohsaka
JOURNAL OF MOLECULAR RECOGNITION, 29, 10, 485-491, 2016
◆Misc
遺伝暗号とタンパク質機能の拡張
芳坂 貴弘
高分子, 63(10), 722-723-, 2014
非天然アミノ酸のタンパク質への導入技術－バイオメディカル応用に向けて
芳坂 貴弘
メディカルバイオ別冊, 72-77-, 2010
Artificial Protein Synthesizing System Using Extended Genetic Code
HOHSAKA Takahiro
Seibutsu Butsuri, 47, 2, 124-128, 2007
CHEMICAL BIOLOGY(9)遺伝暗号を拡張した人工タンパク質合成システム
芳坂 貴弘
現代化学, 430, 58-62, 2007
遺伝暗号を拡張した人工タンパク質合成システム
芳坂 貴弘
メディカル・サイエンス・ダイジェスト, 2007, 33(3), 744-745., -, 2007
◆Books
第６章変異導入法，揺らぎ・ダイナミクスと生体機能（寺嶋正秀編）
共著, 91-102, 化学同人, 2013
人工タンパク質の合成と応用、超分子サイエンス＆テクノロジー(国武豊喜監修)
共著, p890-895, エヌティーエス, 2009., 2010
非天然型アミノ酸を含むタンパク質, トコトンやさしいタンパク質の本(東京工業大学生命理工学研究科編)
共著, 日刊工業新聞社, 2007., 2007
非天然アミノ酸導入，生物工学ハンドブック(日本生物工学会編)
共著, コロナ社, 2005
人工タンパク質,高分子新素材のすべて(国武豊喜監修)
共著, p138-141, 工業調査会, 2005
◆Conference Activities & Talks
非天然アミノ酸の導入によるタンパク質機能の人工的カスタマイズ
第16回蛋白質科学会年会, 福岡, 2016
非天然アミノ酸導入技術のバイオ医薬への応用展開
第22回ペプチドフォーラム, 金沢, 2016
Incorporation of nonnatural amino acids through expansion of the genetic code and its application to fluorescence analysis of proteins
BMB2015（第38回分子生物学会年会・第88回生化学会大会合同大会）, 神戸, 2015
Incorporation of nonnatural amino acids by expanding the genetic code and its application to fluorescence analysis of proteins
The 14th KIAS Conference on Protein Structure and Function, Korea Institute for Advanced Study, Seoul, Korea, 2014
Site-specific incorporation of fluorescent nonnatural amino acids into proteins and its application to fluorescence analysis of proteins
6th International Symposium on Molecular Science of Fluctuations toward Biological Functions, Kyoto, 2013

■Teaching Experience

Study on Nanobiotechnology with Training Course, Bioscience and Biotechnology(E), Bioorganic Chemistry, ナノバイオテクノロジー論（実習付）, 生命機能工学特論(E), 生物有機化学特論

■Contributions to  Society

◆Academic Society Affiliations
日本生化学会, 高分子学会, 日本化学会, The Molecular Biology Society of Japan, The Japanese Biochemical Society, The Society of Polymer Science, Japan, The Chemical Society of Japan

■Academic  Awards

・ 第17回生物工学論文賞 , 日本生物工学会 , 2009
・ The Young Scientists' Prize, The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology , The Minister of Education, Culture, Sports, Science and Technology , 2007
・ The First Prize, The 4th Japan Biotechnology Business Competition , The Executive Committee of the Japan Biotechnology Business Competition , 2004