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HOHSAKA, Takahiro Professor, Director of Bioscience, Biotechnology, and Biomedical Engineering Research Area
Materials Science, Bioscience, Biotechnology, and Biomedical Engineering

Published Papers

58 items
Antigen-responsive fluorescent antibody probes generated by selective N-terminal modification of IgGs
Fukunaga Keisuke, Watanabe Takayoshi, Novitasari Dian, Ohashi Hiroyuki, Abe Ryoji, Hohsaka Takahiro
CHEMICAL COMMUNICATIONS, 54, 90, 12734-12737, 2018
Antigen-dependent fluorescence response of anti-c-Myc Quenchbody studied by molecular dynamics simulations
Yoshiharu Mori, Hisashi Okumura, Takayoshi Watanabe, Takahiro Hohsaka
Chemical Physics Letters, 698, 223-226, 2018
Statistical description of the denatured structure of a single protein, staphylococcal nuclease, by FRET analysis
Mariko Yamaguchi, Emi Ohta, Takuya Muto, Takayoshi Watanabe, Takahiro Hohsaka, Yoichi Yamazaki, Hironari Kamikubo, Mikio Kataoka
Biophysical Reviews, 10, 2, 145-152, 2018
Freezing-Assisted Gene Delivery Combined with Polyampholyte Nanocarriers
Sana Ahmed, Tadashi Nakaji-Hirabayashi, Takayoshi Watanabe, Takahiro Hohsaka, Kazuaki Matsumura
ACS BIOMATERIALS SCIENCE & ENGINEERING, 3, 8, 1677-1689, 2017
Novel biosensor system model based on fluorescence quenching by a fluorescent streptavidin and carbazole-labeled biotin
Xianwei Zhu, Hiroaki Shinohara, Ryuta Miyatake, Takahiro Hohsaka
JOURNAL OF MOLECULAR RECOGNITION, 29, 10, 485-491, 2016
Antibody-based fluorescent and fluorescent ratiometric indicators for detection of phosphotyrosine
Kim Phuong Huynh Nhat, Takayoshi Watanabe, Kensuke Yoshikoshi, Takahiro Hohsaka
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122, 2, 146-154, 2016
Double-Fluorescent-Labeled Single-Chain Antibodies Showing Antigen-Dependent Fluorescence Ratio Change
Kensuke Yoshikoshi, Takayoshi Watanabe, Takahiro Hohsaka
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 89, 5, 573-580, 2016
Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates
Atsushi Yamaguchi, Takayoshi Matsuda, Kazumasa Ohtake, Tatsuo Yanagisawa, Shigeyuki Yokoyama, Yoshihisa Fujiwara, Takayoshi Watanabe, Takahiro Hohsaka, Kensaku Sakamoto
BIOCONJUGATE CHEMISTRY, 27, 1, 198-206, 2016
Liposome-Based in Vitro Evolution of Aminoacyl-tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation
Atsuko Uyeda, Takayoshi Watanabe, Yasuhiko Kato, Hajime Watanabe, Tetsuya Yomo, Takahiro Hohsaka, Tomoaki Matsuura
CHEMBIOCHEM, 16, 12, 1797-1802, 2015
A novel molecular biosensor system model designed by using a couple composed of a fluorescent mutant binding protein and fluorophore-labeled ligand analogue based on the FRET mechanism
Xianwei Zhu, Hiroaki Shinohara, Takahiro Hohsaka
ANALYTICAL METHODS, 7, 23, 9957-9962, 2015
A Cell-Free Translocation System Using Extracts of Cultured Insect Cells to Yield Functional Membrane Proteins
Toru Ezure, Kei Nanatani, Yoko Sato, Satomi Suzuki, Keishi Aizawa, Satoshi Souma, Masaaki Ito, Takahiro Hohsaka, Gunnar von Heijine, Toshihiko Utsumi, Keietsu Abe, Eiji Ando, Nobuyuki Uozumi
PLOS ONE, 9, 12, e112874-, 2014
Incorporation of glycosylated amino acid into protein by an in vitro translation system
Teruhiko Matsubara, Kazutoshi Iijima, Takayoshi Watanabe, Takahiro Hohsaka, Toshinori Sato
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 23, 20, 5634-5636, 2013
Incorporation of Fluorescent Nonnatural Amino Acid into Sialic Acid-Binding Lectin for Fluorescence Detection of Ligand-Binding
Yasutaka Ito, Takahiro Hohsaka
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 86, 6, 729-735, 2013
Amber codon-mediated expanded saturation mutagenesis of proteins using a cell-free translation system
Naoki Shozen, Takayoshi Watanabe, Takahiro Hohsaka
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113, 6, 704-709, 2012
Synthesis of Novel BRET/FRET Protein Probes Containing Light-Emitting Proteins and Fluorescent Nonnatural Amino Acids
Atsushi Yamaguchi, Takahiro Hohsaka
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 85, 5, 576-583, 2012
Incorporation of unnatural non-α-amino acids into the N-terminus of proteins in a cell-free translation system
Takahiro Hohsaka
Methods in Molecular Biology, 794, 229-239, 2012
"Quenchbodies": Quench-Based Antibody Probes That Show Antigen-Dependent Fluorescence
Ryoji Abe, Hiroyuki Ohashi, Issei Iijima, Masaki Ihara, Hiroaki Takagi, Takahiro Hohsaka, Hiroshi Ueda
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133, 43, 17386-17394, 2011
Farnesyl pyrophosphate regulates adipocyte functions as an endogenous PPAR gamma agonist
Tsuyoshi Goto, Hiroyuki Nagai, Kahori Egawa, Young-Il Kim, Sota Kato, Aki Taimatsu, Tomoya Sakamoto, Shogo Ebisu, Takahiro Hohsaka, Hiroh Miyagawa, Shigeru Murakami, Nobuyuki Takahashi, Teruo Kawada
BIOCHEMICAL JOURNAL, 438, 1, 111-119, 2011
Biosynthesis of proteins containing modified lysines and fluorescent labels using non-natural amino acid mutagenesis
Yasunori Tokuda, Takayoshi Watanabe, Kazushi Horiike, Kaori Shiraga, Ryoji Abe, Norihito Muranaka, Takahiro Hohsaka
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111, 4, 402-407, 2011
Incorporation of fluorescent non-natural amino acids into N-terminal tag of proteins in cell-free translation and its dependence on position and neighboring codons
Ryoji Abe, Kaori Shiraga, Shogo Ebisu, Hiroaki Takagi, Takahiro Hohsaka
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 110, 1, 32-38, 2010
Incorporation of Fluorescent-Labeled Non-alpha-Amino Carboxylic Acids into the N-Terminus of Proteins in Response to Amber Initiation Codon
Masanori Miura, Norihito Muranaka, Ryoji Abe, Takahiro Hohsaka
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 83, 5, 546-553, 2010
Incorporation of Non-Natural Amino Acids with Two Labeling Groups into the N-Terminus of Proteins
Tadayoshi Egusa, Kaori Shiraga, Takuya Chiba, Yasunori Tokuda, Issei Iijima, Takahiro Hohsaka
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 83, 2, 176-181, 2010
Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis
Naoki Shozen, Issei Iijima, Takahiro Hohsaka
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19, 17, 4909-4911, 2009
Position-Specific Incorporation of Fluorescent Non-natural Amino Acids into Maltose-Binding Protein for Detection of Ligand Binding by FRET and Fluorescence Quenching
Issei Iijima, Takahiro Hohsaka
CHEMBIOCHEM, 10, 6, 999-1006, 2009
Screening of amber suppressor tRNAs suitable to introduce nonnatural amino acids into proteins by real-time monitoring of cell-free translation.
Iijima I, Hohsaka T
Nucleic acids symposium series (2004), 53, 297-298, 2009
Comprehensive screening of amber suppressor tRNAs suitable for incorporation of non-natural amino acids in a cell-free translation system
Hikaru Taira, Yosuke Matsushita, Kenji Kojima, Kaori Shiraga, Takahiro Hohsaka
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 374, 2, 304-308, 2008
N-terminal specific fluorescence labeling of proteins through incorporation of fluorescent hydroxy acid and subsequent ester cleavage
Takayoshi Watanabe, Yoichi Miyata, Ryoji Abe, Norihito Muranaka, Takahiro Hohsaka
CHEMBIOCHEM, 9, 8, 1235-1242, 2008
Four-base codon-mediated saturation mutagenesis in a cell-free translation system
Takayoshi Watanabe, Norihito Muranaka, Takahiro Hohsaka
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 105, 3, 211-215, 2008
Position-specific incorporation of biotinylated non-natural amino acids into a protein in a cell-free translation system
Takayoshi Watanabe, Norihito Muranaka, Issel Iijima, Takahlro Hohsaka
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 361, 3, 794-799, 2007
Incorporation of unnatural non-alpha-amino acids into the N terminus of proteins in a cell-free translation system
Norihito Muranaka, Masanori Miura, Hikaru Taira, Takahiro Hohsaka
CHEMBIOCHEM, 8, 14, 1650-1653, 2007
BIOL 45-Expansion of translation initiation: Incorporation of non-alpha-amino acids into the N-terminus of proteins by using expanded initiation codons in a cell-free translation system
Norihito Muranaka, Masanori Miura, Hikaru Taira, Takahiro Hohsaka
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 234, -, 2007
Incorporation of nonnatural amino acids into proteins in cell-free translation systems
Takahiro Hohsaka
SEIKAGAKU, 79, 3, 247-253, 2007
N-terminal specific fluorescence labeling of proteins through fourbase codon-mediated incorporation of fluorescent hydroxy acid.
Watanabe T, Miyata Y, Abe R, Muranaka N, Hohsaka T
Nucleic acids symposium series (2004), 51, 363-364, 2007
FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids
Daisuke Kajihara, Ryoji Abe, Issei Iijima, Chie Komiyama, Masahiko Sisido, Takahiro Hohsaka
NATURE METHODS, 3, 11, 923-929, 2006
pH-Sensitive locomotion of cycloclextrins in a block-selective mobile polyrotaxane
Hak Soo Choi, Ayaka Hirasawa, Tooru Ooya, Daisuke Kajihara, Takahiro Hohsaka, Nobuhiko Yui
CHEMPHYSCHEM, 7, 8, 1671-1673, 2006
Binding efficiency of elongation factor Tu to tRNAs charged with nonnatural fluorescent amino acids
H Nakata, T Ohtsuki, R Abe, T Hohsaka, M Sisido
ANALYTICAL BIOCHEMISTRY, 348, 2, 321-323, 2006
Four-base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids
N Muranaka, T Hohsaka, M Sisido
NUCLEIC ACIDS RESEARCH, 34, 1, e7-, 2006
In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system
H Taira, T Hohsaka, M Sisido
NUCLEIC ACIDS RESEARCH, 34, 5, 1653-1662, 2006
Development of amber suppressor tRNAs appropriate for incorporation of nonnatural amino acids.
Taira H, Matsushita Y, Kojima K, Hohsaka T
Nucleic acids symposium series (2004), 50, 233-234, 2006
Molecular design of glycoprotein mimetics: Glycoblotting by engineered proteins with an oxylamino-functionalized amino acid residue
N Matsubara, K Oiwa, T Hohsaka, R Sadamoto, K Niikura, N Fukuhara, A Takimoto, H Kondo, S Nishimurar
CHEMISTRY-A EUROPEAN JOURNAL, 11, 23, 6974-6981, 2005
Four-base codon/anticodon strategy and non-enzymatic aminoacylation for protein engineering with non-natural amino acids
M Sisido, K Ninomiya, T Ohtsuki, T Hohsaka
METHODS, 36, 3, 270-278, 2005
A small-molecule-based approach to sense codon-templated natural-unnatural hybrid peptides. Selective silencing and reassignment of the sense codon by orthogonal reacylation stalling at the single-codon level
S Sando, K Kanatani, N Sato, H Matsumoto, T Hohsaka, Y Aoyama
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 127, 22, 7998-7999, 2005
Synthesis and sequence optimization of GFP mutants containing aromatic non-natural amino acids at the Tyr66 position
D Kajihara, T Hohsaka, M Sisido
PROTEIN ENGINEERING DESIGN & SELECTION, 18, 6, 273-278, 2005
Four-base codon-mediated incorporation of nonnatural amino acids into proteins in a eukaryotic cell-free translation system
H Taira, M Fukushima, T Hohsaka, M Sisido
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99, 5, 473-476, 2005
Efficient synthesis of nonnatural mutants in Escherichia coli S30 in vitro protein synthesizing system
K Yamanaka, H Nakata, T Hohsaka, M Sisido
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97, 6, 395-399, 2004
Position-specific incorporation of dansylated non-natural amino acids into streptavidin by using a four-base codon
T Hohsaka, N Muranaka, C Komiyama, K Matsui, S Takaura, R Abe, H Murakami, M Sisido
FEBS LETTERS, 560, 1-3, 173-177, 2004
Site-directed incorporation of non-natural amino acid into Streptomyces xylanase.
Ichikawa T, Kuno A, Taki M, Hohsaka T, Sisido M, Kaneko S, Taira K, Kobayashi H, Hasegawa T
Nucleic acids symposium series (2004), 48, 161-162, 2004
Facile aminoacylation of pdCpA dinucleotide with a nonnatural amino acid in cationic micelle.
Ninomiya K, Kurita T, Hohsaka T, Sisido M
Chemical communications (Cambridge, England), 17, 2242-2243, 2003
Random insertion and deletion mutagenesis.
Murakami H, Hohsaka T, Sisido M
Methods in molecular biology (Clifton, N.J.), 231, 53-64, 2003
Incorporation of fluorescently labeled nonnatural amino acids into proteins in an E. coli in vitro translation system.
Hohsaka T, Abe R, Shiraga K, Sisido M
Nucleic acids research. Supplement (2001), 3, 271-272, 2003
Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs
M Taki, T Hohsaka, H Murakami, K Taira, M Sisido
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 124, 49, 14586-14590, 2002
Incorporation of non-natural amino acids into proteins
T Hohsaka, M Sisido
CURRENT OPINION IN CHEMICAL BIOLOGY, 6, 6, 809-815, 2002
Preparation of glycosylated amino acid derivatives for glycoprotein synthesis by in vitro translation system
S Manabe, K Sakamoto, Y Nakahara, M Sisido, T Hohsaka, Y Ito
BIOORGANIC & MEDICINAL CHEMISTRY, 10, 3, 573-581, 2002
Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs
H Murakami, T Hohsaka, M Sisido
NATURE BIOTECHNOLOGY, 20, 1, 76-81, 2002
Photoswitching of peroxidase activity by position-specific incorporation of a photoisomerizable non-natural amino acid into horseradish peroxidase
N Muranaka, T Hohsaka, M Sisido
FEBS LETTERS, 510, 1-2, 10-12, 2002
Aminoacylation of tRNA by antisense molecule.
Ninomiya K, Endo T, Minohata T, Tajiri M, Suzuki M, Kurita T, Hohsaka T, Sisido M
Nucleic acids research. Supplement (2001), 2, 101-102, 2002
Nonnatural mutagenesis in E. coli and rabbit reticulocyte lysates by using four-base codons.
Hohsaka T, Fukushima M, Sisido M
Nucleic acids research. Supplement (2001), 2, 201-202, 2002
A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin.
Taki M, Hohsaka T, Murakami H, Kuno A, Hasegawa T, Taira K, Sisido M
Nucleic acids research. Supplement (2001), 2, 203-204, 2002