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OHKI, Shinya Professor, Director of Center for Nano Materials and Technology
Materials Science, Bioscience, Biotechnology, and Biomedical Engineering, Center for Nano Materials and Technology

Published Papers

60 items
Zinc finger domains bind low-complexity domain polymers
Naohiko Iguchi, Noriyoshi Isozumi, Yoshikazu Hattori, Tomohiro Imamura, Masatomo So, Hitoki Nanaura, Takao Kiriyama, Nobuyuki Eura, Minako Yamaoka, Mari Nakanishi, Masashi Mori, Shinya Ohki, Hiroyuki Kumeta, Hironori Koga, Mai Watabe, Takuya Mabuchi, Shingo Kanemura, Masaki Okumura, Takuya Yoshizawa, Ichiro Ota, Naoki Suzuki, Masashi Aoki, Yoshito Yamashiro, Tomohide Saio, Kazuma Sugie, Eiichiro Mori
-, 2023
Mutation-driven evolution of antibacterial function in an ancestral antifungal scaffold: Significance for peptide engineering
Jing Gu, Noriyoshi Isozumi, Bin Gao, Shinya Ohki, Shunyi Zhu
Frontiers in Microbiology, 13, -, 2022
Structural and functional studies of LaIT2, an antimicrobial and insecticidal peptide from Liocheles australasiae
Maiki Tamura, Chiharu Tatsushiro, Eugene Hayato Morita, Shinya Ohki
Toxicon, 214, 8-17, 2022
Red-Beet Betalain Pigments Inhibit Amyloid-β Aggregation and Toxicity in Amyloid-β Expressing Caenorhabditis elegans
Tomohiro Imamura, Noriyoshi Isozumi, Yasuki Higashimura, Hironori Koga, Tenta Segawa, Natsumi Desaka, Hiroki Takagi, Kenji Matsumoto, Shinya Ohki, Masashi Mori
Plant Foods for Human Nutrition, 77, 1, 90-97, 2022
Adaptively evolved human oral actinomyces‐sourced defensins show therapeutic potential
Shunyi Zhu, Bin Gao, Yoshitaka Umetsu, Steve Peigneur, Ping Li, Shinya Ohki, Jan Tytgat
EMBO Molecular Medicine, -, 2021
Fungal effector SIB1 of Colletotrichum orbiculare has unique structural features and can suppress plant immunity in Nicotiana benthamiana
Ru Zhang, Noriyoshi Isozumi, Masashi Mori, Ryuta Okuta, Suthitar Singkaravanit-Ogawa, Tomohiro Imamura, Jun-Ichi Kurita, Pamela Gan, Ken Shirasu, Shinya Ohki, Yoshitaka Takano
Journal of Biological Chemistry, 297, 6, 101370-101370, 2021
Evolution-Based Protein Engineering for Antifungal Peptide Improvement
Jing Gu, Noriyoshi Isozumi, Shouli Yuan, Ling Jin, Bin Gao, Shinya Ohki, Shunyi Zhu
Molecular Biology and Evolution, -, 2021
Similarities and differences in the conformational stability and reversibility of ORF8, an accessory protein of SARS-CoV-2, and its L84S variant
Shinya Ohki, Tomohiro Imamura, Yasuki Higashimura, Kenji Matsumoto, Masashi Mori
Biochemical and Biophysical Research Communications, 563, 92-97, 2021
Overexpression and purification of a toxic peptide LaIT2 from Japanese scorpion, Liocheles australasiae
Maiki Tamura, Chiharu Tatsushiro, Eugene Hayato Morita, Shinya Ohki
Protein Expression and Purification, 182, 105835-105835, 2021
Structure and antimicrobial activity of NCR169, a nodule-specific cysteine-rich peptide of Medicago truncatula
Noriyoshi Isozumi, Yuya Masubuchi, Tomohiro Imamura, Masashi Mori, Hironori Koga, Shinya Ohki
Scientific Reports, 11, 1, 9923-9923, 2021
Benzopyrylium salts as new anticancer, antibacterial, and antioxidant agents
Swayamsiddha Kar, Abdul Akhir, Sidharth Chopra, Shinya Ohki, Balasubramanyam Karanam, Nageswara Rao Golakoti
Medicinal Chemistry Research, https://doi.org/10.1007/s00044-020-02685-3-, 2021
Production of ORF8 protein from SARS-CoV-2 using an inducible virus-mediated expression system in suspension-cultured tobacco BY-2 cells
Tomohiro Imamura, Noriyoshi Isozumi, Yasuki Higashimura, Shinya Ohki, Masashi Mori
Plant Cell Reports, https://doi.org/10.1007/s00299-020-02654-5, -, 2021
A novel WD40-repeat protein involved in formation of epidermal bladder cells in the halophyte quinoa
Tomohiro Imamura, Yasuo Yasui, Hironori Koga, Hiroki Takagi, Akira Abe, Kanako Nishizawa, Nobuyuki Mizuno, Shinya Ohki, Hiroharu Mizukoshi, Masashi Mori
Communications Biology, 3, 1, 513-513, 2020
Met872 is the key residue determining the novel binomial binding of metabotropic glutamate receptor 7 to calmodulin
Isozumi Noriyoshi, Ohki Shinya
Biochem. Biophys. Res. Commun., 520, 640-644, 2019
Ca2+-dependent interaction between calmodulin and CoDN3, an effector of Colletotrichum orbiculare
Isozumi Noriyoshi, Inoue Yoshihiro, Imamura Tomohiro, Mori Masashi, Takano Yoshitaka, Ohki Shinya
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 514, 3, 803-808, 2019
Isolation of amaranthin synthetase from Chenopodium quinoa and construction of an amaranthin production system using suspension-cultured tobacco BY-2 cells.
Tomohiro Imamura, Noriyoshi Isozumi, Yasuki Higashimura, Akio Miyazato, Hiroharu Mizukoshi, Shinya Ohki, Masashi Mori
Plant biotechnology journal, 17, 5, 969-981, 2019
Isolation and characterization of the betalain biosynthesis gene involved in hypocotyl pigmentation of the allotetraploid Chenopodium quinoa
Tomohiro Imamura, Hiroki Takagi, Akio Miyazato, Shinya Ohki, Hiroharu Mizukoshi, Masashi Mori
Biochemical and Biophysical Research Communications, 496, 2, 280-286, 2018
Chirality-dependent interaction of D- and L-menthol with biomembrane models
Pooja Gusain, Shinya Ohki, Kunihide Hoshino, Yoshio Tsujino, Naofumi Shimokawa, Masahiro Takagi
Membranes, 7, 4, -, 2017
Three-dimensional structure of the complex between calmodulin mutant lacking the c-terminal five residues and the calmodulin-binding peptide derived from skeletal muscle myosin light-chain kinase
Vu Van Dung, Yoshitaka Umetsu, Shinya Ohki
TAP CHI SINH HOC, 39, 3, 309-319, 2017
Mesomartoxin, a new K(v)1.2-selective scorpion toxin interacting with the channel selectivity filter
Xueli Wang, Yoshitaka Umetsu, Bin Gao, Shinya Ohki, Shunyi Zhu
BIOCHEMICAL PHARMACOLOGY, 93, 2, 232-239, 2015
Structure of the Microtubule-Binding Domain of Flagellar Dynein
Yusuke S. Kato, Toshiki Yagi, Sarah A. Harris, Shin-Ya Ohki, Kei Yura, Youské Shimizu, Shinya Honda, Ritsu Kamiya, Stan A. Burgess, Masaru Tanokura
Structure, 22, 11, 1628-1638, 2014
Unique Structural Changes in Calcium-Bound Calmodulin Upon Interaction with Protein 4.1R FERM Domain: Novel Insights into the Calcium-dependent Regulation of 4.1R FERM Domain Binding to Membrane Proteins by Calmodulin
Wataru Nunomura, Noriyoshi Isozumi, Shigeyoshi Nakamura, Yuji Jinbo, Shinya Ohki, Shun-ichi Kidokoro, Hideki Wakui, Yuichi Takakuwa
CELL BIOCHEMISTRY AND BIOPHYSICS, 69, 1, 7-19, 2014
Central Cell-Derived Peptides Regulate Early Embryo Patterning in Flowering Plants
Liliana M. Costa, Eleanor Marshall, Mesfin Tesfaye, Kevin A. T. Silverstein, Masashi Mori, Yoshitaka Umetsu, Sophie L. Otterbach, Ranjith Papareddy, Hugh G. Dickinson, Kim Boutiller, Kathryn A. VandenBosch, Shinya Ohki, Jose F. Gutierrez-Marcos
SCIENCE, 344, 6180, 168-172, 2014
Experimental Conversion of a Defensin into a Neurotoxin: Implications for Origin of Toxic Function
Shunyi Zhu, Steve Peigneur, Bin Gao, Yoshitaka Umetsu, Shinya Ohki, Jan Tytgat
MOLECULAR BIOLOGY AND EVOLUTION, 31, 3, 546-559, 2014
1P032 Production and NMR study of plant defensin-like peptides using plant cell expression system(Protein: Structure & Function,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))
Yoshitaka Umetsu, Masashi Mori, Shinya Ohki
Seibutsu Butsuri, 54, 1, S146-, 2014
Novel Mechanism of Regulation of Protein 4.1G Binding Properties Through Ca2+/Calmodulin-Mediated Structural Changes
Wataru Nunomura, Yuji Jinbo, Noriyoshi Isozumi, Shinya Ohki, Yoshinobu Izumi, Norio Matsushima, Yuichi Takakuwa
Cell Biochemistry and Biophysics, 66, 3, 545-558, 2013
Evolutionary Relationship and Structural Characterization of the EPF/EPFL Gene Family
Naoki Takata, Kiyonobu Yokota, Shinya Ohki, Masashi Mori, Toru Taniguchi, Manabu Kurita
PLoS ONE, 8, 6, -, 2013
3P091 Toward the establishment of an expression system for actin(01F. Protein: Engineering,Poster)
Mori Masashi, Umetsu Yoshitaka, Ohki Shinya
Seibutsu Butsuri, 53, 1, S227-, 2013
Overexpression of an antimicrobial peptide derived from C. elegans using an aggregation-prone protein coexpression system
Satoshi Tomisawa, Eri Hojo, Yoshitaka Umetsu, Shinya Ohki, Yusuke Kato, Mitsuhiro Miyazawa, Mineyuki Mizuguchi, Masakatsu Kamiya, Yasuhiro Kumaki, Takashi Kikukawa, Keiichi Kawano, Makoto Demura, Tomoyasu Aizawa
AMB EXPRESS, 3, -, 2013
Structural Characterization of a Trapped Folding Intermediate of Pyrrolidone Carboxyl Peptidase from a Hyperthermophile
Mineyuki Mizuguchi, Makoto Takeuchi, Shinya Ohki, Yuko Nabeshima, Takahide Kouno, Tomoyasu Aizawa, Makoto Demura, Keiichi Kawano, Katsuhide Yutani
BIOCHEMISTRY, 51, 31, 6089-6096, 2012
Alternative splicing produces structural and functional changes in CUGBP2
Hitoshi Suzuki, Makoto Takeuchi, Ayumu Sugiyama, A. H. M. Khurshid Alam, Luyen Thi Vu, Yoshiharu Sekiyama, Hieu Chi Dam, Shin-ya Ohki, Toshifumi Tsukahara
BMC BIOCHEMISTRY, 13, 6-, 2012
Synthesis of well-defined hyperbranched polymers bio-based on multifunctional phenolic acids and their structure-thermal property relationships
Siqian Wang, Seiji Tateyama, Daisaku Kaneko, Shin-ya Ohki, Tatsuo Kaneko
POLYMER DEGRADATION AND STABILITY, 96, 12, 2048-2054, 2011
The NMR structure of stomagen reveals the basis of stomatal density regulation by plant peptide hormones
Shinya Ohki, Makoto Takeuchi, Masashi Mori
NATURE COMMUNICATIONS, 2, -, 2011
Identification of the functional domain of stomagen, a positive signal of stomata development
Sugano Shigeo S, Takeuchi Makoto, Shimada Tomoo, Ohki Shinya, Mori Masashi, Hara-Nishimura Ikuko
Plant and Cell Physiology Supplement, 2011, 0, 196-196, 2011
Conformation of the calmodulin-binding domain of metabotropic glutamate receptor subtype 7 and its interaction with calmodulin
Noriyoshi Isozumi, Yoshinori Iida, Akiko Nakatomi, Nobuaki Nemoto, Michio Yazawa, S. Ohki
JOURNAL OF BIOCHEMISTRY, 149, 4, 463-474, 2011
Roles of the C-terminal residues of calmodulin in structure and function
Chihiro Kitagawa, Akiko Nakatomi, Dasol Hwang, Issey Osaka, Hiroki Fujimori, Hideya Kawasaki, Ryuichi Arakawa, Yota Murakami, Shinya Ohki
Biophysics, 7, 35-49, 2011
Expression and purification of metabotropic glutamate receptor 7 peptides
Noriyoshi Isozumi, S. Ohki
PROTEIN EXPRESSION AND PURIFICATION, 73, 1, 46-50, 2010
Solution structure of the inhibitory phosphorylation domain of myosin phosphatase targeting subunit 1
Shunsuke Mori, Ryou Iwaoka, Masumi Eto, Shin-ya Ohki
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 77, 3, 732-735, 2009
Stable-isotope labeling using an inducible viral infection system in suspension-cultured plant cells
Shinya Ohki, Koji Dohi, Atsushi Tamai, Makoto Takeuchi, Masashi Mori
JOURNAL OF BIOMOLECULAR NMR, 42, 4, 271-277, 2008
Stable isotope labeling methods for protein NMR spectroscopy
Shin-Ya Ohki, Masatsune Kainosho
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 53, 4, 208-226, 2008
Effect of Parallel Processing and Optimization Techniques in Molecular Dynamics
Kenji Satou, Kenri Konno, Osamu Ohta, Kazunori Mikami, Keita Teranishi, Yoichi Yamada, Shin-Ya Ohki
PROCEEDINGS OF THE 1ST WSEAS INTERNATIONAL CONFERENCE ON BIOMEDICAL ELECTRONICS AND BIOMEDICAL INFORMATICS, 228-+, 2008
Phosphorylation-induced conformational switching of CPI-17 produces a potent myosin phosphatase inhibitor
Masumi Eto, Toshio Kitazawa, Fumiko Matsuzawa, Sei-Ichi Aikawa, Jason A. Kirkbride, Noriyoshi Isozumi, Yumi Nishimura, David L. Brautigan, Shin-Ya Ohki
STRUCTURE, 15, 12, 1591-1602, 2007
Structural insight into regulation of myosin phosphatase by CPI-17.
Masumi Eto, Toshio Kitazawa, Furniko Matsuzawa, Sei-ichi Aikawa, Jason A. Kirkbride, Noriyoshi Isozumi, Yumi Nishimura, David L. Brautigan, Shin-ya Ohki
FASEB JOURNAL, 21, 5, A520-A520, 2007
Tautomerism of histidine 64 associated with proton transfer in catalysis of carbonic anhydrase
Hideto Shimahara, Takuya Yoshida, Yasutaka Shibata, Masato Shimizu, Yoshimasa Kyogoku, Fumo Sakiyama, Takashi Nakazawa, Shin-ichi Tate, Shin-ya Ohki, Takeshi Kato, Hozumi Moriyama, Ken-ichi Kishida, Yasuo Tano, Tadayasu Ohkubo, Yuji Kobayashi
JOURNAL OF BIOLOGICAL CHEMISTRY, 282, 13, 9646-9656, 2007
Structural basis for calcium-regulated relaxation of striated muscles at interaction sites of troponin with actin and tropomyosin
Kenji Murakami, Fumiaki Yumoto, Shin-ya Ohki, Takuo Yasunaga, Masaru Tanokura, Takeyuki Wakabayashi
REGULATORY MECHANISMS OF STRIATED MUSCLE CONTRACTION, 592, 71-86, 2007
Comparative analysis of protein thermo stability: Differences in amino acid content and substitution at the surfaces and in the core regions of thermophilic and mesophilic proteins
Kiyonobu Yokota, Kenji Satou, Shin-ya Ohki
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS, 7, 3, 255-262, 2006
Structural basis for Ca2+-regulated muscle relaxation at interaction sites of troponin with actin and tropomyosin
K Murakami, F Yumoto, S Ohki, T Yasunaga, M Tanokura, T Wakabayashi
JOURNAL OF MOLECULAR BIOLOGY, 352, 1, 178-201, 2005
Al3+ interaction sites of calmodulin and the Al3+ effect on target binding of calmodulin
H Kurita, A Nakatomi, H Shimahara, M Yazawa, S Ohki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 333, 4, 1060-1065, 2005
Computational simulation for interactions of nano-molecules: The phospho-pivot modeling algorithm for prediction of interactions between a phospho-protein and its receptor
Fumiko Matsuzawa, Shin-Ya Ohki, Sei-Ich Aikawa, Masumi Eto
Science and Technology of Advanced Materials, 6, 5, 463-467, 2005
Phospho-pivot modeling predicts specific interactions of protein phosphatase-1 with a phospho-inhibitor protein CPI-17
F Matsuzawa, S Aikawa, S Ohki, M Eto
JOURNAL OF BIOCHEMISTRY, 137, 5, 633-641, 2005
2P156 Structural basis for Ca2+-regulated muscle relaxation at interaction sites of troponin with actin and tropomyosin
Murakami K., Yumoto F., Ohki S., Yasunaga T., Tanokura M., Wakabayashi T.
Seibutsu Butsuri, 45, S158-, 2005
2P086 Analysis of factors responsible for enhanced thermostability of protein : systematical analysis
Yokota K., Sato K., Ohki S.
Seibutsu Butsuri, 45, S141-, 2005
2P072 NMR investigation of the refolding intermediate of pyrrolidone carboxyl peptidase from a hyperthermophile
Takeuchi M., Mizuguchi M., Kouno T., Ohki S., Yutani K., Mori Y., Shinoda H., Kawano K.
Seibutsu Butsuri, 45, S137-, 2005
1P050 Interaction between calmodulin and A13+
Ohki S., Kurita H., Nakatomi A., Shimahara H., Yazawa M.
Seibutsu Butsuri, 45, S44-, 2005
Phosphorylation-induced conformational change responsible for the function of a myosin phosphatase inhibitor, CPI-17
Shin-ya Ohki, Masumi Eto, Rei Takada, Masato Shimizu, David L. Brautigan, Masatsune Kainosho
Science and Technology of Advanced Materials, 5, 3, 383-386, 2004
1P139 Structural basis of myosin phosphatase inhibitor, CPI-17
Ohki S., Eto M., Yokota K., Matsuzawa F.
Seibutsu Butsuri, 44, S64-, 2004
1P148 Structural basis for Ca^<2+>-sensitive muscle relaxation at actin-binding sites of troponin
Murakami K., Yumoto F., Ohki S., Yasunaga T., Tanokura M., Wakabayashi T.
Seibutsu Butsuri, 44, S66-, 2004
Distinctive solution conformation of phosphatase inhibitor CPI-17 substituted with aspartate at the phosphorylation-site threohine residue
SY Ohki, M Eto, M Shimizu, R Takada, DL Brautigan, M Kainosho
JOURNAL OF MOLECULAR BIOLOGY, 326, 5, 1539-1547, 2003
[Developing a new approach for high-throughput, high-accuracy NMR structural analyses of genomic proteins].
Tsutomu Terauchi, Shin-Ya Ohki, Masatsune Kainosho
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 47, 8 Suppl, 1045-51, 2002
Solution NMR structure of the myosin phosphatase inhibitor protein CPI-17 shows phosphorylation-induced conformational changes responsible for activation
Shin-Ya Ohki, Masumi Eto, Eri Kariya, Toshiya Hayano, Yuichiro Hayashi, Michio Yazawa, David Brautigan, Masatsune Kainosho
Journal of Molecular Biology, 314, 4, 839-849, 2001